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KMID : 0613820030130020191
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Journal of Life Science
2003 Volume.13 No. 2 p.191 ~ p.196
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Transcription level of the ars-1 promoter of Neurospora crassa
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Lee Bheong-Uk
Koo Sang-Ho
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Abstract
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The ars gene of the Neurospora crassa encodes arylsulfatase and is expressed under sulfur limitation. An ars-1 promoter (Pars) translationally-fused to a lacZ gene was transformed into the N. crassa RLM 35-35, a his-3 inl strain and integrated into the his-3 locus by a single crossover homologous recombination. β-galactosidase specific activity was measured from mycelia grown in sulfur-limited Vogel’s medium. Enzyme activity reached its maximum at 14 hour after the shift to derepressing condition. When activity from homokaryon generated by microconidiation was measured, it was 17% higher than that from heterokaryon.
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KEYWORD
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Neurospora crassa, ars-1, arylsulfatase, promoter strength
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